Study Material

HISTORY & TYPES OF PAPER CHROMATOGRAPHY

HISTORY In 1850 Runge use the process called “capillary analysis” for separating inorganic mixtures on various types of papers. However paper chromatography was not established until at late 1940s. In 1944 Congden, Gorden, Martin and Synge was able to separate amino acids by using papers. At present. paper chromatography is the method in which the analysis of an unknown substance ...

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CHROMATOGRAPHIC TECHNIQUES

CHROMATOGRAPHIC TECHNIQUES A brief description on the historical development of the main techniques is listed below: 1:    PAPER CHROMATOGRAPHY In this method, the dissolved substances are applied as a small spot on a cellulose bound filter paper which is then kept in a container dipped in an organic solvent. The mixture are partitioned between paper (Stationary phase) and organic solvent (mobile ...

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CHROMATOGRAPHIC METHODS

CHROMATOGRAPHIC METHODS                       In all the chromatographic techniques difference in affinity involves the process of either adsorption or partition. In adsorption the bonding of a compound to the surface of the solid phase takes place. In case of partition, the relative solubility of a compound in two phases results in the ...

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Discuss Renaissance prose,who were its main authors?

  Renaissance – roughly 1485-1660 The most known/influential authors of Renaissance prose were: – Sir Francis Bacon o Essays o The Advancement of Learning o Novum Organum o New Atlantis – Richard Hooker o Laws of Ecclesiastical Polity – Sir Thomas More o Utopia – Sir Walter Raleigh o The Discovery of Guiana o The History of the World – ...

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Rf VALUE (RETENTION FACTOR)

WHAT IS Rf  VALUE (RETENTION FACTOR) The movement of any substance relative to the solvent front in a given chromatographic system is constant characteristic of that substance. In paper chromatography Rf value is constant, Rf =  Distance of spot of component from the original line          _________________________________________            Distance of solvent front from ...

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Define adsorption, development, chromatogram & resolution

  The physical attachment of the substances with other material is called adsorption. In a separation of mixtures by paper chromatography or thin layer chromatography, the production of coloured derivatives of the solutes by spraying the stationary phase with selective reagents in order to establish the location of individual substances is called development. The finished paper or thin layer plate ...

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What is elution, eluent & eluate?

Definitions of elution, eluent & eluate? The removal of adsorbed species from a porous bed or chromatographic column by means of a stream of liquid or gas is called elution. The liquid used to extract one material from another in chromatography is called eluent. The product which emerges from the column that is eluent and whatever component of the test sample ...

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What is Stationary phase and Mobile phase?

What is Stationary phase and Mobile phase? The phase which remains fixed by a support or does not move by the incoming mobile phase is called stationary phase. The mixture of solvent which moves through the paper or silica gel (The substance present as inert support such as silica gel or paper) to separate the sample into its components (The ...

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DEFINITION & HISTORY OF CHROMATOGRAPHY

 DEFINITION & HISTORY OF CHROMATOGRAPHY   Chromatography is a modem technique used for the examination and separation of mixture of chemical substances. It facilitates the purification, isolation and comparison of compounds. It may be employed with all kinds of volatile and soluble substances, organic and inorganic, polar and non polar, and it may he adopted to use with various quantities ...

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What is Chromatography

  DEFINITION OF CHROMATOGRAPHY Chromatography is a modem technique used for the examination and separation of mixture of chemical substances. It facilitates the purification, isolation and comparison of compounds. It may be employed with all kinds of volatile and soluble substances, organic and inorganic, polar and non polar, and it may he adopted to use with various quantities under many ...

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