1. One of the major advantages of paper chromatography is the sensitivity with which compounds can be located after separation. Amounts as little as 0.1 microgram (one micrograms is one millionth of a gram) of some compounds can be detected with routine reagents.
2. The operation time is small.
DISADVANTAGES OF PAPER CHROMATOGRAPHY
1. Paper chromatographic techniques can not be used in separation of volatile substances such as hydrocarbons and volatile fatty acids. 2. The lower limit for the detection of most compounds is 1-5 microgram.