There are a number of applications of uv-visible spectroscopy. Some of the important applications are given below:
(1)IDENTIFICATION OF A COMPOUND:
The identification of a synthetic compound can be established by comparing its spectrum with the spectra of known standard samples
(2)QUANTITATIVE ESTIMATION OF A COMPOUND
Ultraviolet visible spectroscopy can be used for quantitative analysis using Beer-lambert law. The concentration of any compound with a known molar absorptivity can be readily calculated from the measured absorbance of the sample.
UV-visible spectroscopy provides a good method for the determination of the rates of many reactions which involve a change in an absorbing group. The rate of a reaction can be measured by following either the decrease in absorbance (if the reactant is the absorbing species) or the increase in absorbance (if the product is the absorbing species) as a function of time.
(4)STUDY OF CHEMICAL EQUILIBRIA IN SOLUTION
The UV- visible region has been employed for studying a wide variety of chemical equilibria such as acid-base equilibria, tautomerie equilibria and complex formation.
(5)STEREO- CHEMICAL STUDIES
The UV-Visible spectroscopy can also be used for studying stereo-chemical details of certain molecules. For example hindered rotation in orthosubstituted biphenyls can be readily studied by uv Spectroscopy. In case of geometrical or cis-trans isomerism, the trans isomer shows absorption maximum at a longer wavelength than the cis-isomer.