Principle & Types of Papers in Paper Chromatography

PRINCIPLE The use of paper as a chromatographic medium is usually regarded as a typically partition system, where the stationary phase is water, held by adsorption on cellulose molecules, which in turn are kept in a fixed position by the fibrous structure of the paper. It is now realized that the paper used for the inert support of the mobile ...

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HISTORY In 1850 Runge use the process called “capillary analysis” for separating inorganic mixtures on various types of papers. However paper chromatography was not established until at late 1940s. In 1944 Congden, Gorden, Martin and Synge was able to separate amino acids by using papers. At present. paper chromatography is the method in which the analysis of an unknown substance ...

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CHROMATOGRAPHIC TECHNIQUES A brief description on the historical development of the main techniques is listed below: 1:    PAPER CHROMATOGRAPHY In this method, the dissolved substances are applied as a small spot on a cellulose bound filter paper which is then kept in a container dipped in an organic solvent. The mixture are partitioned between paper (Stationary phase) and organic solvent (mobile ...

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CHROMATOGRAPHIC METHODS                       In all the chromatographic techniques difference in affinity involves the process of either adsorption or partition. In adsorption the bonding of a compound to the surface of the solid phase takes place. In case of partition, the relative solubility of a compound in two phases results in the ...

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 DEFINITION & HISTORY OF CHROMATOGRAPHY   Chromatography is a modem technique used for the examination and separation of mixture of chemical substances. It facilitates the purification, isolation and comparison of compounds. It may be employed with all kinds of volatile and soluble substances, organic and inorganic, polar and non polar, and it may he adopted to use with various quantities ...

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