CHROMATOGRAPHIC METHODS                      

In all the chromatographic techniques difference in affinity involves the process of either adsorption or partition.

In adsorption the bonding of a compound to the surface of the solid phase takes place.

In case of partition, the relative solubility of a compound in two phases results in the partition of compound. Thus all types of chromatography known so far have been grouped in either of two forms.




I. Partition column chromatography.

  1. Paper chromatography. .

  2. Thin layer chromatography (TLC).

  3. Gel filtration.


  1. Adsorption column chromatography

  2. Thin layer chromatography

  3. Ion exchange chromatography


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