Definition & History of Thin layer chromatography



Definition & History of Thin layer chromatography

Thin layer chromatography (TLC) or planer chromatography, began as a method of separating mixtures by eluting them through a planner chromatographic bed theh visualizing the separated components by staining or charting.

For the first time Beyerinck in 1889 and Wijsmann in 1896 worked on TLC. After that lzmailow and Shraiber in l938 gave the ideas and fundamental principles of using a chromatographic adsorbent in the form of a thin layer fixed on an inert rigid support. Meinhard in 1949 developed an open column and in 1950 Kirchner reported the separation of teipenes on a “chromatostrip“ prepared by coating 21 small glass strip with an adsorbent mixed with starch or plaster of Paris which acted as a binder. In the late 1950s Stahl showed that thin layer chromatography could he applied to a wide variety of separations. It was by his
hard work which was based on theory and practice that thin layer chromatography has occupied a unique position amongst all the chromatographic techniques. TLC is now regarded as an indispensable tool in both quality control and research laboratories. The technique is easy to learn fast, versatile and is preferred to other techniques such as gas chromatography and high performance liquid chromatography.

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