What is Chromatography



Chromatography is a modem technique used for the examination and separation of mixture of chemical substances. It facilitates the purification, isolation and comparison of compounds. It may be employed with all kinds of volatile and soluble substances, organic and inorganic, polar and non polar, and it may he adopted to use with various quantities under many different conditions.

According to the¬†International Union of Pure and Applied Chemistry (IUPAC), in 1993 Chromatography is defined as, “A method for the separation of components of a sample, in which the components are distributed between two phases, one of which is stationary while the other moves in definite direction. The stationary phase may be a solid or a liquid supported on a solid or gel. The mobile phase may be gaseous or liquid.




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